abstract：:The inhibition of rat liver monoamine oxidase by a number of N-propargyl and alpha-methyl amine derivatives has been examined. The results indicate that alpha-methyl-substituted primary and secondary amine derivatives tend to show selectivity as reversible inhibitors towards the A-form of the enzyme. The structural fe...

journal_title：Biochemical pharmacology

authors： Tipton KF,McCrodden JM,Kalir AS,Youdim MB

更新日期：1982-04-01 00:00:00

abstract：:Bernserazide (D,L-serine 2-[2,3,4-trihydroxybenzyl]-hydrazide) as been shown to inhibit the clorgyline-resistant amine oxidase (CRAO) activities which metabolize benzylamine in homogenates of rat aorta, heart and brown adipose tissue. In vitro studies showed a concentration- and time-dependent inhibition of CRAO in he...

journal_title：Biochemical pharmacology

authors： Lyles GA,Callingham BA

更新日期：1982-04-01 00:00:00

abstract：:The experiments were designed to investigate some details of the action of 3-methylcholanthrene (3-MC) on the regulation of transcription. After a single intraperitoneal dose of 3-MC a significant increase in the activities of both nucleolar and nucleoplasmic protein kinases in hepatic cells of young rats was found. T...

journal_title：Biochemical pharmacology

authors： Kleeberg U,Szeberényi J,Juhasz P,Tigyi A,Klinger W

更新日期：1982-03-15 00:00:00

abstract：:The effects of propranolol or phentolamine on the metabolism of phospholipids, diacylglycerol, and triacylglycerol were studied in the bovine retina in vitro. Lipid labeling was followed during short-term incubation of intact bovine retinas with [U-14C]glycerol and [1-14C]palmitic acid. Each of these precursors was re...

journal_title：Biochemical pharmacology

authors： de Boschero MG,Bazan NG

更新日期：1982-03-15 00:00:00

abstract：:Cobalt protoporphyrin generated from 5-amino[4-14C]laevulinate by homogenates or primary cultures of chick embryo liver exposed to CoCl2 was found to be radioactivity unextractable by acid/acetone, when extra protein was added. The activity of ferrochelatase was required for formation of cobalt protoporphyrin since in...

journal_title：Biochemical pharmacology

authors： Sinclair PR,Sinclair JF,Bonkowsky HL,Gibbs AH,De Matteis F

更新日期：1982-03-15 00:00:00

abstract：:Treatment of rats with carbon tetrachloride (CCl4) resulted in early reproducible losses of either one or two specific polypeptides (depending on the inducing agent with which the animals had been treated) in the molecular weight range of the multiple forms of cytochrome P-450. The loss was correlated with a decrease ...

journal_title：Biochemical pharmacology

authors： Noguchi T,Fong KL,Lai EK,Olson L,McCay PB

更新日期：1982-03-01 00:00:00

abstract：:(1) As a first step in studying the kinetics of the lipolytic system of rat adipocytes, the day-to-day variation between dose-response curves has been analysed. (2) Methods are described for the evaluation of large quantities of data relating noradrenaline to lipolysis. (3) A 'clustering' technique is presented which ...

journal_title：Biochemical pharmacology

authors： Davies JI,Cooper DM,Everett D

更新日期：1982-03-01 00:00:00

abstract：:The effects of the transmembrane pH gradient (delta pH) and the transmembrane potential gradient (delta psi) on the uptake of several sympathomimetic amines were investigated, using bovine adrenal chromaffin granules isolated in isotonic sucrose. As previously described [R. Johnson and A. Scarpa, J. Biol. Chem. 254 37...

journal_title：Biochemical pharmacology

authors： Johnson RG,Carty SE,Hayflick S,Scarpa A

更新日期：1982-03-01 00:00:00

abstract：:A serine peptidase (RLK1) was partially purified from rat liver homogenates. Its molecular weight was 80,000, and its optimum pH was 7.5. Bz-Tyr-O-Et was hydrolyzed by the enzyme, which was inhibited by Ip2PF, PMSF and by Tos-Phe-CH2Cl. The bonds cleaved by the enzyme were Phe5-Ser6 and Phe8-Arg9, when bradykinin was ...

journal_title：Biochemical pharmacology

authors： Da Graça M,Mazzacoratti N,Sampaio CA

更新日期：1982-03-01 00:00:00

abstract：:We employed both [5-3H] ara-C and ([2-14C] ara-C labeled L1210 DNA for analysis following exposure to alkali under various conditions. The results demonstrated that the tritium label on C5 of ara-C molecules incorporated in DNA was exchanged with water under alkaline conditions and, therefore, radioactivity was subseq...

journal_title：Biochemical pharmacology

authors： Major PP,Egan EM,Herrick D,Kufe DW

更新日期：1982-03-01 00:00:00

abstract：:Approximately 60% of the total particulate phosphodiesterase activity occurring in cardiac tissue was associated with the nuclear fraction. Cyclic GMP phosphodiesterase activity of the purified cardiac nuclear fraction was selectively inhibited by trifluoperazine (I50 = 19 microM) with negligible inhibition (less than...

journal_title：Biochemical pharmacology

authors： Ahluwalia GS,Rhoads AR

更新日期：1982-03-01 00:00:00

abstract：:Rat liver microsomes and purified NADPH-cytochrome c reductase metabolized [14C]misonidazole anaerobically to a reactive intermediate that covalently binds to tissue macromolecules. Air strongly inhibited the binding whereas carbon monoxide had no effect, indicating that misonidazole is activated via reduction and not...

journal_title：Biochemical pharmacology

authors： McManus ME,Lang MA,Stuart K,Strong J

更新日期：1982-02-15 00:00:00

abstract：:The widely used muscarinic receptor ligand [3H]quinuclidinyl benzilate ([3H]QNB) was found to bind in a site-specific but artifactual manner to rat intestinal mucus, obscuring specific binding to muscarinic receptors on intestinal epithelial cells. Atropine inhibited [3H]QNB binding to mucus with an apparent IC50 of 2...

journal_title：Biochemical pharmacology

authors： Rimele TJ,Gaginella TS

更新日期：1982-02-15 00:00:00

abstract：:The relative ease of generation of reactive oxygen species from a series of reductively activated aglycone and sugar modified anthracyclines was compared by the extents of single strand scission in supercoiled PM2-covalently closed circular (CCC)-DNA. The electrochemical properties of these agents were used as a quant...

journal_title：Biochemical pharmacology

authors： Lown JW,Chen HH,Plambeck JA,Acton EM

更新日期：1982-02-15 00:00:00

abstract：:The substrate specificity of highly purified pig liver catechol-O-methyltransferase has been investigated kinetically. This enzyme shows stereospecificity towards the naturally occurring L-isomer of 3,4-dihydroxyphenylalanine (DOPA) which has a higher affinity and maximal velocity as a substrate than the D-form. We ha...

journal_title：Biochemical pharmacology

authors： Gordonsmith RH,Raxworthy MJ,Gulliver PA

更新日期：1982-02-01 00:00:00

abstract：:A cyclic nucleotide phosphodiesterase from guinea-pig heart is activated by calmodulin in the presence of calcium ions. Activation was measured over a range of calmodulin concentrations, and is antagonised by several tricyclic psychotropic drugs including trifluoperazine, imipramine, chlorpromazine and amitriptyline. ...

journal_title：Biochemical pharmacology

authors： Reynolds CH,Claxton PT

更新日期：1982-02-01 00:00:00

abstract：:Catalase, superoxide dismutase, and dimethylsulfoxide were tested for their ability to prevent the cytotoxic effect of 6-hydroxydopamine (6-OHDA) on the human neuroblastoma line SY5Y. Viability was measured at two time points after 6-OHDA treatment: at 3 hr by means of amino acid incorporation and at 24 hr by trypan b...

journal_title：Biochemical pharmacology

authors： Tiffany-Castiglioni E,Saneto RP,Proctor PH,Perez-Polo JR

更新日期：1982-01-15 00:00:00

abstract：:Mammary gland and liver microsomes of lactating rats were examined for the components of mixed function oxidase and related enzyme activities. Cytochrome b5, NADH- and NADPH- dependent cytochrome c reductase activities were 15-, 6- and 10-fold lower, respectively, in the mammary gland than in the liver microsomes. The...

journal_title：Biochemical pharmacology

authors： Ritter CL,Malejka-Giganti D

更新日期：1982-01-15 00:00:00

abstract：:These in vitro studies indicate that N-oxidation of N-hydroxyamphetamine (NOHA) by rat liver homogenates yields phenylacetone oxime (PAOx) as the major metabolite. This oxidation was NADPH and oxygen dependent but was not appreciably increased in microsomes from phenobarbital-pretreated animals. The addition to micros...

journal_title：Biochemical pharmacology

authors： Matsumoto RM,Cho AK

更新日期：1982-01-01 00:00:00

abstract：:With the aid of a chiral derivatizing reagent and a sensitive and specific nitrogen-phosphorus gas chromatographic assay, metabolism of para-chloroamphetamine (PCA) enantiomers has been studied following incubation of racemic (RS)-, R(-)- and S(+)-PCA with rabbit liver microsomal preparations. Significant metabolism o...

journal_title：Biochemical pharmacology

authors： Ames MM,Frank SK

更新日期：1982-01-01 00:00:00

abstract：:Antitubulin activities of ansamitocins, maytansine and four maytansinoids which are structurally related to ansamitocins were studied using three reaction systems; inhibition of polymerization of bovine brain tubulin, depolymerization of the once polymerized tubulin, and immunofluorescent staining of cytoplasmic micro...

journal_title：Biochemical pharmacology

authors： Ikeyama S,Takeuchi M

更新日期：1981-09-01 00:00:00

abstract：:The dimeric forms of metallothionein were isolated from livers of rabbits which accumulated cadmium in large quantities. The dimers were separated into three isoproteins, both on a DEAE Sephadex A-25 column and on a gel permeation column. Reduction of each dimer with mercaptoethanol yielded monomeric metallothionein-I...

journal_title：Biochemical pharmacology

authors： Suzuki KT,Yamamura M

更新日期：1980-03-01 00:00:00

abstract：:Incubation of rat peritoneal mast cells with hydrogen peroxide results in a marked release of histamine. Maximal release is observed with 0.05-0.1 mM H(2)O(2), but higher concentrations of H(2)O(2) instead suppresses the release. Histamine release starts after about 2 min of lag time and reaches a plateau in about 10 ...

journal_title：Biochemical pharmacology

authors： Ohmori H,Yamamoto I,Akagi M,Tasaka K

更新日期：1980-03-01 00:00:00

abstract：:Cytidine deaminase, an enzyme found in the supernatant fluid of hepatocytes, granulocytes and tumor cells, and in plasma, degrades the antitumor agents cytosine arabinoside and 5-azacytidine. Uridine and its analogs, 3-deazauridine, 5-bromodeoxyuridine, 5-fluorodeoxyuridine and 6-azauridine, were found to competitivel...

journal_title：Biochemical pharmacology

authors： Drake JC,Hande KR,Fuller RW,Chabner BA

更新日期：1980-03-01 00:00:00

abstract：:Developmental correlation of higher levels of estrogen binding by macromolecules in rat liver supernatant and of increases in plasma renin substrate levels after estrogen administration is reported. Gel filtration columns were used to separate bound from free radioactivity in studying binding of radioactive estradiol...

journal_title：Biochemical pharmacology

authors： Eisenfeld AJ,Krakoff LR,Aten RF

更新日期：1977-05-15 00:00:00